HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Gregor Zünd René Prêtre Urs Niederhäuser Paul Vogt Marko Turina Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zünd, G. Articles by Turina, M. Search for Related Content PubMed PubMed Citation Articles by Zünd, G. Articles by Turina, M.

Eur J Cardiothorac Surg 1998;13:293-297
© 1998 Elsevier Science NL

Endothelial cell injury in cardiac surgery: salicylate may be protective by reducing expression of endothelial adhesion molecules¹

^a Clinic for Cardio-vascular Surgery, University Hospital, Rämistrasse 100, CH-8091 Zurich, Switzerland
^b Center for Experimental Therapeutics and Reperfusion Injury, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA

Received 29 September 1997; received in revised form 3 December 1997; accepted 9 December 1997.

Corresponding author. Tel.: +41 1 2551111; fax: +41 1 2554369; e-mail: zund@chi.usz.ch

Objective: Cardiac surgery with cardiopulmonary bypass induces ischemia to the heart, hypoxemia to various tissues and release of endotoxins. The endothelial cell may suffer from hypoxia and trigger cascades of adverse reactions by activation of neutrophils through adhesion molecules. The authors measured expression of intercellular adhesion molecule-1 (ICAM-1), during hypoxia and normoxia and hypothesized that salicylate, which inhibits the nuclear factor-B (NFB), an hypoxia-dependent transmission factor, could reduce this expression. Methods: Human umbilical vein endothelial cells were cultured and exposed to normoxia and hypoxia in the presence of lipopolysaccharide (LPS). The endothelial cells were thereafter treated with salicylate or indomethacin under the same conditions. The surface expression of ICAM-1 was measured by whole cell enzyme-linked immunosorbent assay (ELISA) and the NFB expression by Western blotting. Results: In the presence of LPS and under hypoxic conditions, the endothelial cells produced a 300±41% increased expression of ICAM-1 compared with normoxia. The addition of salicylate (0.02–20 mM) completely inhibited the enhanced expression of ICAM-1, the addition of indomethacin at equivalent concentrations did not reduce ICAM-1 expression under either condition. Conclusion: ICAM-1 expression is greatly enhanced by the hypoxic endothelial cell in the presence of circulating endotoxin. Pre-treatment with salicylate completely abolishes the enhanced expression. The study suggests that salicylate administered before cardiopulmonary bypass might protect the heart against ischemic/reperfusion injuries and reduce the load of the overall inflammatory reaction.

Key Words: Endothelium • Ischemia/reperfusion injury • Treatment

This article has been cited by other articles:

				M Buyukates, S Acikgoz, O Kandemir, E Aktunc, E Ceylan, and M Can Use of warm priming solution in open heart surgery: its effects on hemodynamics and acute inflammation Perfusion, March 1, 2008; 23(2): 89 - 94. [Abstract] [PDF]

				U. P. Schurr, G. Zund, S. P. Hoerstrup, J. Grunenfelder, F. E. Maly, P. R. Vogt, and M. I. Turina Preoperative administration of steroids: influence on adhesion molecules and cytokines after cardiopulmonary bypass Ann. Thorac. Surg., October 1, 2001; 72(4): 1316 - 1320. [Abstract] [Full Text] [PDF]

				C. H. Selzman, S. A. Miller, and A. H. Harken Therapeutic implications of inflammation in atherosclerotic cardiovascular disease Ann. Thorac. Surg., June 1, 2001; 71(6): 2066 - 2074. [Abstract] [Full Text] [PDF]

				C. A. Labarrere, D. R. Nelson, S. J. Miller, J. M. Nieto, J. A. Conner, D. E. Pitts, P. C. Kirlin, and H. G. Halbrook Value of Serum-Soluble Intercellular Adhesion Molecule-1 for the Noninvasive Risk Assessment of Transplant Coronary Artery Disease, Posttransplant Ischemic Events, and Cardiac Graft Failure Circulation, September 26, 2000; 102(13): 1549 - 1555. [Abstract] [Full Text] [PDF]

				J. Grunenfelder, G. Zund, A. Schoeberlein, E.R. Schmid, U. Schurr, R. Frisullo, F. Maly, and M. Turina Expression of adhesion molecules and cytokines after coronary artery bypass grafting during normothermic and hypothermic cardiac arrest Eur. J. Cardiothorac. Surg., June 1, 2000; 17(6): 723 - 728. [Abstract] [Full Text] [PDF]

				J. Grunenfelder, G. Zund, A. Schoeberlein, F. E. Maly, U. Schurr, S. Guntli, K. Fischer, and M. Turina Modified ultrafiltration lowers adhesion molecule and cytokine levels after cardiopulmonary bypass without clinical relevance in adults Eur. J. Cardiothorac. Surg., January 1, 2000; 17(1): 77 - 83. [Abstract] [Full Text] [PDF]

				T. Stefanec Circulating Apoptotic Endothelial Cells Blood, August 15, 1999; 94(4): 1482 - 1483. [Full Text] [PDF]