HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Kerem M. Vural Mehmet C. Oz Hasan F. Batirel David J. Pinsky Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vural, K. M. Articles by Pinsky, D. J. Search for Related Content PubMed PubMed Citation Articles by Vural, K. M. Articles by Pinsky, D. J.

Eur J Cardiothorac Surg 1999;16:150-155
© 1999 Elsevier Science NL

Membrane stabilization in harvested vein graft storage: effects on adhesion molecule expression and nitric oxide synthesis

^a Department of Cardiothoracic Surgery, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
^b Department of Physiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
^c Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
^d Department of Cardiovascular Surgery, Yuksek Ihtisas Hospital of Turkey, Istanbul, Turkey
^e Department of Thoracic Surgery, Marmara University, Istanbul, Turkey

Corresponding author. N. Tandogan cad., 5/6 Kavaklidere, 06540 Ankara, Turkey. Tel.: +90-312-4267574; fax: +90-312-4266181
e-mail: kvural{at}tr-net.net.tr

Objective: Expression of cellular adhesion molecules in human saphenous vein grafts may occur even during harvesting and storage, before the grafts have been implanted as bypass conduits. This may play a role in graft adaptation to arterial flow conditions, which may play an important role in late graft patency. In this study, ketotifen, a mast cell membrane stabilizing agent was studied for its effects on reducing endothelial reactivity during storage of harvested vein graft segments. Methods: Human saphenous vein grafts, obtained from seven patients and then divided into two equal parts of control and study specimens, were stored in either heparinized blood (Group A) or heparinized blood containing 100 µg/ml ketotifen (Group B) for 1 h at room temperature. Specimens were analyzed by Western blotting to quantify ICAM-1, E-selectin, P-selectin, VCAM-1, and inducible nitric oxide synthase (NOS-2) expression, as well as tissue cGMP levels in response to topical application of an endothelium-independent vasodilator. Results: ICAM-1, E-selectin and P-selectin expression did not differ between the groups. However, VCAM-1 expression was significantly lower in Group B (460±29 vs. 289±50, P=0.01). NOS-2 expression (488±64 vs. 577±38, P=0.02) and tissue cGMP levels (2.2±0.6 pmol/ml vs. 5.7±1.7 pmol/ml, P=0.01) in response to nitroglycerin (24±10% vs. 11±5%, P=0.02) were higher in Group B. Conclusions: Of all of the adhesion receptors studied, only VCAM-1 expression was reduced by a mast cell membrane-stabilizing agent, perhaps because of activation of the venous endothelium during harvest prior to ketotifen exposure. However, ketotifen also augmented NOS-2 expression, increased tissue cGMP levels in response to nitroglycerin. These actions may improve vascular homeostasis in the venous graft, suggesting the possibility that this strategy may improve long-term graft patency.

Key Words: Saphenous • Vein graft • Adhesion molecule • Reoperation • Membrane stabilizer • Nitric oxide

This article has been cited by other articles:

				K. Hinokiyama, G. Valen, S. Tokuno, J. B. Vedin, and J. Vaage Vein graft harvesting induces inflammation and impairs vessel reactivity. Ann. Thorac. Surg., October 1, 2006; 82(4): 1458 - 1464. [Abstract] [Full Text] [PDF]

				S. Kaplan, J. A. Morgan, G. Bisleri, F. H. Cheema, H. O. Akman, V. K. Topkara, and M. C. Oz Effects of Resveratrol in Storage Solution on Adhesion Molecule Expression and Nitric Oxide Synthesis in Vein Grafts Ann. Thorac. Surg., November 1, 2005; 80(5): 1773 - 1778. [Abstract] [Full Text] [PDF]

				T. Sakaguchi, T. Asai, D. Belov, M. Okada, D. J. Pinsky, A. M. Schmidt, and Y. Naka Influence of ischemic injury on vein graft remodeling: Role of cyclic adenosine monophosphate second messenger pathway in enhanced vein graft preservation J. Thorac. Cardiovasc. Surg., January 1, 2005; 129(1): 129 - 137. [Abstract] [Full Text] [PDF]

				M. P Vallely, P. G Bannon, C. F Hughes, and L. Kritharides Endothelial Cell Adhesion Molecules and Cardiopulmonary Bypass Asian Cardiovasc Thorac Ann, December 1, 2001; 9(4): 349 - 355. [Abstract] [Full Text] [PDF]