| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Eur J Cardiothorac Surg 2001;19:156-163
© 2001 Elsevier Science NL
Second Department of Surgery, Fukui Medical University, 23-3 Shimoaizuki, Matsuoka-Cho, Yoshida-Gun, Fukui 9101193, Japan
Received 31 August 2000; received in revised form 20 November 2000; accepted 4 December 2000.
Corresponding author. Tel.: +81-776-61-8379; fax: 81-776-61-8114
e-mail: riweiw{at}fmsrsa.fukui-med.ac.jp
Objective: Transmyocardial laser revascularization (TMLR) has been widely evaluated as a treatment for ischemic myocardium. However, its mechanism remains unclear. One mechanism is angiogenesis. This study examines the relationship between TMLR and angiogenesis from the viewpoint of matrix metalloproteinases and platelet-derived endothelial cell growth factor. Methods: The left anterior descending coronary artery (LAD) was ligated permanently in 12 beagle dogs. TMLR was accomplished in six of the 12 dogs using a carbon dioxide laser. No laser treatment was done in the six control dogs. Two weeks after the initial operation, dogs were euthanized and transmural samples (each of approximately 0.5 g) were cut from the center of the infarcted LAD territory, right ventricular wall, left circumflex artery perfuse area and interventricular septum except the LAD perfuse area. They were snap-frozen in liquid nitrogen for matrix metalloproteinases and platelet-derived endothelial cell growth factor activity analysis. Hematoxylin and eosin staining, double immunohistologic staining with anti-proliferating cell nuclear antigen and von Willebrand factor antibody, and immunohistologic staining with antibody against platelet-derived endothelial cell growth factor were performed for histologic studies. The activities of matrix metalloproteinases were examined by gelatin zymography. The activity of platelet-derived endothelial cell growth factor was examined by a spectrophotometric method. Results: The channels were found to be infiltrated with granulation tissue and fibrosis. In the laser group, the active matrix metalloproteinase-2 and platelet-derived endothelial cell growth factor activity in the area of the left anterior descending coronary artery was significantly higher than in the control group (P<0.0001 and P=0.037, respectively). Within the channel remnants or close to these areas, the number of von Willebrand factor positive microvessels and proliferating cell nuclear antigen with correlating von Willebrand factor positive microvessels were significantly higher than in the control group (P=0.001 and P=0.0006, respectively). These increases in microvessels significantly correlated with the expression of matrix metalloproteinases and platelet-derived endothelial cell growth factor. Conclusion: Based on these findings it was concluded that transmyocardial laser revascularization induced angiogenesis correlated with the expression of active matrix metalloproteinases-2 and platelet-derived endothelial cell growth factor.
Key Words: Transmyocardial laser revascularization • Angiogenesis • Matrix metalloproteinase • Platelet-derived endothelial cell growth factor
This article has been cited by other articles:
|
|
 |
|
  K. A. Horvath and Y. Zhou Transmyocardial Laser Revascularization and Extravascular Angiogenetic Techniques to Increase Myocardial Blood Flow Card. Surg. Adult, January 1, 2008; 3(2008): 733 - 752. [Full Text]
|
|
|
|
 |
|
 D. Spiegelstein, C. Kim, Y. Zhang, G. Li, R. D. Weisel, R.-K. Li, and T. M. Yau Combined transmyocardial revascularization and cell-based angiogenic gene therapy increases transplanted cell survival Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3311 - H3316. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tasse and R. Arora Transmyocardial Revascularization: Peril and Potential Journal of Cardiovascular Pharmacology and Therapeutics, March 1, 2007; 12(1): 44 - 53. [Abstract] [PDF]
|
|
|
|
 |
|
 W. Li, K. Tanaka, K. Morioka, T. Uesaka, N. Yamada, A. Takamori, M. Handa, S. Tanabe, and A. Ihaya Thymidine Phosphorylase Gene Transfer Inhibits Vascular Smooth Muscle Cell Proliferation by Upregulating Heme Oxygenase-1 and p27KIP1 Arterioscler Thromb Vasc Biol, July 1, 2005; 25(7): 1370 - 1375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Horvath, C. Y. J. Lu, E. Robert, G. F. Pierce, R. Greene, B. A. Sosnowski, and J. Doukas Improvement of myocardial contractility in a porcine model of chronic ischemia using a combined transmyocardial revascularization and gene therapy approach J. Thorac. Cardiovasc. Surg., May 1, 2005; 129(5): 1071 - 1077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Li, K. Tanaka, A. Ihaya, Y. Fujibayashi, S. Takamatsu, K. Morioka, M. Sasaki, T. Uesaka, T. Kimura, N. Yamada, et al. Gene therapy for chronic myocardial ischemia using platelet-derived endothelial cell growth factor in dogs Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H408 - H415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Li, K. Tanaka, Y. Chiba, T. Kimura, K. Morioka, T. Uesaka, A. Ihaya, M. Sasaki, T. Tsuda, and N. Yamada Role of MMPs and plasminogen activators in angiogenesis after transmyocardial laser revascularization in dogs Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H23 - H30. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Huikeshoven, J. F. Beek, J. A.P. van der Sloot, R. Tukkie, J. van der Meulen, and M. J.C. van Gemert 35 years of experimental research in transmyocardial revascularization: what have we learned? Ann. Thorac. Surg., September 1, 2002; 74(3): 956 - 970. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ANN THORAC SURG | ASIAN CARDIOVASC THORAC ANN | EUR J CARDIOTHORAC SURG |
| J THORAC CARDIOVASC SURG | ICVTS | ALL CTSNet JOURNALS |
