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Eur J Cardiothorac Surg 2001;20:187-194
© 2001 Elsevier Science NL

Functional assessment of non-heart-beating donor lungs: prediction of post-transplant function

^a Department of Surgery, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK
^b Comparative Biology Centre, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK

Received 10 October 2000; received in revised form 12 March 2001; accepted 30 March 2001.

Corresponding author. William Leech Centre, Freeman Hospital, High Heaton, Newcastle upon Tyne, NE7 7DN, UK. Tel.: +44-191-284-3111, ext. 26169; fax: +44-191-223-1152
e-mail: j.d.aitchison{at}ncl.ac.uk

Objectives: To enable an increase in the numbers of donor lungs using organs from non-heart-beating donors (NHBD). To develop an isolated ventilation and perfusion technique to assess the degree of warm ischaemic organ injury suffered prior to retrieval, thereby enabling identification of lungs with predictably good post-transplant function. Methods: Lungs from Landrace–Yorkshire White cross pigs were retrieved after 1 (NHBD₁), 2 (NHBD₂) or 4 h (NHBD₄) post-hypoxic death induced by cessation of ventilation. Control organs were retrieved using standard techniques for each group from matched animals immediately following aortic cross-clamping (Control₁ and combined Control_2,4). Modified Euro–Collins pulmoplegia was used in all groups, prior to ventilating a single lung with 100% oxygen and perfusion with neutrophil-depleted and deoxygenated blood. For all of the lungs in the NHBD₂ and combined Control_2,4 groups, and one of the successfully perfused NHBD₄, the contralateral lung was then transplanted with post-transplant function assessed for 12 h. All animals were anaesthetized throughout and euthanased without regaining consciousness. Results: On assessment, oxygenation after 5 min of perfusion did not differ between NHBD₁ (n=4) vs. Control₁ (n=5; analysis of variance (ANOVA), P=0.152). However, oxygenation had deteriorated significantly in the NHBD₂ group (n=6) vs. Control_2,4 (n=8; ANOVA, P<0.0005) and was significantly poorer than initial values after 8 min (unpaired t-test with Bonferroni correction, P<0.03). In NHBD₄ (n=6), four lungs failed assessment due to the development of gross pulmonary oedema, although the remaining pair functioned as well as Control_2,4. Post-transplantation, NHBD₂ (n=6) contralateral lungs showed significantly poorer overall oxygenation, (mean±SD, 46±22 kPa) when compared with Control_2,4 (n=6; 59±16 kPa; ANOVA, P=0.001), although oxygenation was satisfactory. The contralateral organ from one successfully perfused NHBD₄ lung functioned well post-transplantation. Conclusions: The significant deterioration in oxygenating performance seen during assessment after 2 h warm ischaemia and the idiosyncratic function after 4 h warm ischaemia indicates the importance of functional testing of NHBD lungs. The similar deterioration in oxygenating performance seen post-transplantation in the contralateral lungs suggests that this method detects functional warm ischaemic lung injury.

Key Words: Lung transplantation • Non-heart-beating donor • Isolated perfusion • Animal

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