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Eur J Cardiothorac Surg 2003;24:358-363
© 2003 Elsevier Science NL

In vivo model for cross-species porcine endogenous retrovirus transmission using tissue engineered pulmonary arteries

Thorsten Wallesa*, Arthur Lichtenberga, Carmen Puschmannb, Rainer Leyha, Mathias Wilhelmia, Klaus Kallenbacha, Axel Havericha,b, Heike Mertschingb

a Department of Cardiothoracic and Vascular Surgery, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany
b Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Medical School Hannover, Podbielskistrasse 380, D-30659 Hannover, Germany

Received 12 September 2002; received in revised form 24 April 2003; accepted 12 May 2003.

* Corresponding author. Tel.: +49-511-790-6277; fax: +49-511-790-6266
e-mail: walles{at}thg.mh-hannover.de

Objective: Acellularised porcine scaffolds have been successfully used for cardiovascular tissue engineering. However, there is concern about the possibility of porcine endogenous retrovirus (PERV) transmission. In this study we developed an in vivo model for cross-species PERV transmission. Methods: In vitro autologous repopulated porcine pulmonary arteries (n=6) were implanted in sheep in orthotopic position. Blood samples were collected regularly up to 6 months after implantation and tested for PERV by means of polymerase chain reaction and reverse transcriptase-polymerase chain reaction. Explanted tissue engineered pulmonary arteries were tested for PERV sequences. Results: PERV DNA was detectable in acellularised porcine scaffolds. No PERV sequences were detectable 6 months after implantation of in vitro repopulated acellularised porcine pulmonary arteries and in all tested peripheral blood samples. Conclusions: Acellularised porcine matrix scaffolds can be used for cardiovascular tissue engineering of autologous grafts without risk of PERV transmission.

Key Words: Xenotransplantation • Porcine endogenous retrovirus • Tissue engineering • Bioartificial vessel grafts • Cardiovascular




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Copyright © 2003 European Association for Cardio-Thoracic Surgery. Published by Elsevier. All rights reserved.