HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Omar Mangoush Sharif Al-Ruzzeh Permission Requests Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mangoush, O. Articles by Amrani, M. Search for Related Content PubMed PubMed Citation Articles by Mangoush, O. Articles by Amrani, M. Related Collections Cardiac - pharmacology Coronary disease

Eur J Cardiothorac Surg 2003;24:541-546
© 2003 Elsevier Science NL

Effect of ascorbic acid on endothelium-dependent vasodilatation of human arterial conduits for coronary artery bypass grafting

National Heart and Lung Institute, Heart Science Centre, Harefield Hospital, Harefield, Middlesex UB9 6JH, UK

Received 16 March 2003; received in revised form 14 June 2003; accepted 19 June 2003.

^* Corresponding author. Tel.: +44-1895-828550; fax: +44-1895-828002
e-mail: mr.amrani{at}rbh.nthames.nhs.uk

Objective: Techniques aimed at improving the performance of arterial conduits will maximize the clinical benefit achievable with coronary artery bypass surgery. Controlling oxidant stress could be a strategy for preventing early graft deterioration. We tested the effect of a free radical scavenger, ascorbic acid (vitamin C), on preserving the endothelium-dependent vasodilatation function in vitro of radial artery and internal thoracic artery. We also tested its effect on the amount of reactive oxygen species (ROS) generated by each graft. Methods: Radial artery (RA, n=25) and internal thoracic (ITA, n=19) segments were obtained from coronary artery bypass grafting patients. Each segment was divided into 3–4 mm vascular rings and incubated with or without ascorbic acid (10^-3 mol/l) for 1 h or 72 h. Using the organ bath technique, the endothelium-dependent vasodilatation function was tested in vitro by the addition of cumulative concentrations of acetylcholine (10^-9–10^-5 mol/l) following vasocontraction by endothelin-1 (3x10^-8 mol/l). ROS were measured by using chemiluminescence technique at 1-h and after 72 h incubation with or without ascorbic acid. Results: There were no differences in the vasodilatation function between control and ascorbic acid group of both arteries in the 1-hour incubation experiment. However, in the 72 h incubation experiment, ascorbic acid preserved the endothelium-dependent vasodilatation function of RA compared with control group (35.8±2.2% vs. 25.9±2.1%; P=0.005), but not ITA (39±3.5% vs. 40.5±9.3%; P=0.438). After 72 h incubation, RA generated significantly more free radicals compared with 1 h (133.7±151.5 vs. 16.8±16.8 cps/mgx100; P=0.01); however, AA has no statistically significant effect on decreasing the amount of free radicals generated by both arteries. Conclusions: In RA, ascorbic acid is able to preserve the endothelium-dependent vasodilatation function after 72 h incubation, but not after 1 h. However, the mechanism of action of AA is not completely understood. This finding could open the door for understanding the role of oxidant stress and antioxidants in preserving the endothelial function of coronary artery bypass grafts.

Key Words: Vitamin C • Radial artery • Oxidant stress

Abbreviations: AA, ascorbic acid • ACE, angiotensin-converting enzyme • ACh, acetylcholine • CABG, coronary artery bypass grafting • ET-1, endothelin-1 • ITA, internal thoracic artery • RA, radial artery • NO^•, nitric oxide • O₂^•-, superoxide radical • OH^•, hydroxyl radical • ONOO^•, peroxynitrite • SOD, superoxide dismutase • SNP, sodium nitroprusside • CL, chemiluminescence