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Eur J Cardiothorac Surg 2006;30:54-58
© 2006 Elsevier Science NL

IL-10 and TNF-ß gene polymorphisms have no major influence on lactate levels after cardiac surgery

^a Department of Anaesthesiology and Intensive Care Medicine, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic
^b Centre for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
^c Department of Cardiovascular Surgery, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
^d Cardiovascular Research Centre, Prague, Czech Republic

Received 19 September 2005; received in revised form 9 February 2006; accepted 10 February 2006.

^_* Corresponding author. Address: Department of Anaesthesiology and Intensive Care Medicine, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague, Czech Republic. Tel.: +420 261 365 195; fax: +420 261 362 799. (Email: hynek.riha{at}ikem.cz).

Objective: Lactate levels after cardiac surgery are influenced by different proinflammatory (TNF, IL-6, IL-8) and anti-inflammatory (IL-10) cytokines. The goal of the study was to determine the relationship between polymorphism in the IL-10 (–1082G/A) and TNF-ß (+252G/A) genes and lactate levels in patients after cardiac surgery. Methods: We performed prospective observational study in 168 consecutive adult patients without left ventricle dysfunction undergoing elective coronary artery bypass grafting. Lactic acid levels were documented at five different time points: 10 min after beginning of cardiopulmonary bypass, 40 min after cardiopulmonary bypass termination, and 30 min, 8 h, and 16 h after the surgery. Genetic analysis for polymorphism was performed by mismatched polymerase chain reaction and restriction analysis. Results: No association was found between single polymorphism in IL-10 or TNF-ß gene and lactate levels, but the carriers of IL-10/TNF-ß genotype combination +A/GG had significantly different course of lactate levels in time with decrease in lactate (in comparison with increase in other groups) at 8 h after the surgery. Conclusions: IL-10 (–1082G/A) and TNF-ß (+252G/A) gene polymorphisms have a little, yet measurable influence on the time course of changes in lactate levels after cardiac surgery.

Key Words: Cardiopulmonary bypass • Inflammatory response • Genetics • Inflammatory mediators • Lactic acid • Cardiac surgery