HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via Google Scholar Google Scholar Articles by An, Y. Articles by Xiao, Y.-B. Search for Related Content PubMed PubMed Citation Articles by An, Y. Articles by Xiao, Y.-B. Related Collections Cardiac - other Extracorporeal circulation

Eur J Cardiothorac Surg 2007;31:1037-1043. doi:10.1016/j.ejcts.2007.01.077
Copyright © 2007, European Association for Cardio-Thoracic Surgery. Published by Elsevier B.V. All rights reserved

The preventative role of growth hormone on acute liver injury induced by cardiopulmonary bypass in a rat model

Department of Cardiovascular Surgery, Cardiovascular Surgery Center of P.L.A, Xin-Qiao Hospital, Third Military Medical University, ChongQing, China

Received 17 November 2006; received in revised form 22 January 2007; accepted 31 January 2007.

^_* Corresponding author. Address: Department of Cardiovascular Surgery, Cardiovascular Surgery Center of P.L.A, Xin-Qiao Hospital, Third Military Medical University, ChongQing 400037, China. Tel.: +86 23 68755607; fax: +86 23 68755607. (Email: anyongsmcvs{at}163.com).

Objective: Cardiopulmonary bypass (CPB)-induced acute liver injury is a life-threatening complication after cardiac surgery and is thought to be associated with inflammatory response and acute-phase response (APR). Recombinant human growth hormone (rhGH) can modulate the APR and inflammatory response. Here, we tested the protective effect of GH on CPB-induced liver injury in the rat. Methods: Adult male Sprague–Dawley rats (group G, received 2.5 mg/kg of rhGH intramuscularly at 8 a.m. every 24 h for 3 days and just before the initiation of CPB; group C served as control) underwent CPB (120 min, 120 ml/kg per minute, 34 °C) and were killed 3 h after the termination of CPB. Results: Administration of rhGH markedly increased serum insulin-like growth factor (IGF)-I and IGF-I-binding protein (IGFBP)-3 compared with group C. Group G showed significantly lower serum concentrations of alanine aminotransferase and total bilirubin after CPB termination. Those receiving rhGH demonstrated a significant increase in serum prealbumin and serum transferrin and a marked decrease in serum amyloid A and serum C-reactive protein compared with group C. rhGH significantly decreased serum tumor necrosis factor- (TNF-) and interleukin-1ß (IL-1ß), whereas no changes were found for serum IL-6 and IL-10 compared with group C. rhGH significantly increased total liver protein content, hepatocyte proliferation, and decreased hepatocyte apoptosis versus group C. Conclusions: These results suggest that GH administration in rats seems to play a preventative role in acute liver injury associated with CPB via the decrease in acute-phase proteins, proinflammatory cytokines TNF- and IL-1ß, and hepatocyte apoptosis, which is associated with increase in constitutive hepatic proteins, total liver protein content, and hepatocyte proliferation.

Key Words: Growth hormone • Cardiopulmonary bypass • Liver injury • Acute-phase response • Cytokines • Inflammatory response • Rat • Disease model