HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Zhu, J. Articles by Zhang, Y. Search for Related Content PubMed Articles by Zhu, J. Articles by Zhang, Y. Related Collections Cardiac - pharmacology Cardiac - physiology Coronary disease Myocardial infarction

Low dose cyclophosphamide rescues myocardial function from ischemia-reperfusion in rats

^a Department of Cardiology, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, PR China
^b Institute of Cardiology, Zhejiang University, Hangzhou, PR China

Received 28 January 2008; received in revised form 15 May 2008; accepted 15 May 2008.

^_* Corresponding author. Address: Department of Cardiology, the First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, PR China. Tel.: +86 571 87236569; fax: +86 571 87236569. (Email: qiuyuangang1{at}yahoo.com.cn).

Objectives: The effect of low dose of cyclophosphamide (CP) protecting cardiac function from ischemia-reperfusion injury was studied on rats. The premise is that CP inhibits immune and inflammatory process, thereby limits I/R injury and improves myocardial function. Methods: Open chest rats were submitted to 30 min of ischemia followed by 3 h, 12 h or 24 h of reperfusion. Rats were divided into sham group, I/R group and CP group, and each group included 3 time-point subgroups (3 h, 12 h and 24 h; n = 8 for each subgroup). A total of 750 mg/m² cyclophosphamide was intraperitoneally administrated in CP group and saline was given to I/R group. A polyethylene tube was inserted into the left ventricular cavity to detect left ventricular systolic pressure (LVSP) and ±dp/dt _max. At the end of the experiment, hearts were harvested for histopathological assessment and infarct size determination. Results: Compared with I/R group, rats treated with low dose CP showed a significant recovery in myocardial function with improved LVSP (88 ± 11 vs 69 ± 11 mmHg of 3 h; 92 ± 11 vs 64 ± 14 mmHg of 12 h; 90 ± 11 vs 64 ± 14 mmHg of 24 h; p < 0.01 respectively). The ±dp/dt _max also had the similar trends. The myocardial infarct size was reduced in CP group compared to that in I/R group; the infiltration of polymorph nuclear leukocytes (PMNs) in myocardium was decreased in CP group. The histopathological damage score was also attenuated. Conclusions: These findings suggest that low dose CP rescues cardiac function from ischemia-reperfusion injury by alleviating histopathological damage in rat myocardium.

Key Words: Ischemia • Reperfusion • Hemodynamics • Cyclophosphamide • Myocardial stunning