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Biseko^® colloidal solution diminishes the vasoreactivity of human isolated radial arteries

Jen Szolnoky^a, Nóra Ambrus^b, Antal Szabó-Biczók^a, Gábor Bogáts^a, Julius Gy. Papp^b,c, András Varró^b,c, János Pataricza^b,_*

^a Division of Cardiac Surgery, Center for Cardiology, 2nd Department of Internal Medicine, University of Szeged, Szeged, Hungary
^b Department of Pharmacology and Pharmacotherapy, University of Szeged, Szeged, Hungary
^c Division of Cardiovascular Pharmacology, Hungarian Academy of Sciences, Szeged, Hungary

Received 23 June 2008; received in revised form 12 March 2009; accepted 23 March 2009.

^_* Corresponding author. Tel.: +36 62 545 674; fax: +36 62 545 680. (Email: Pataricza{at}phcol.szote.u-szeged.hu).

Objective: Radial arteries are increasingly used as grafts in coronary artery bypass surgery. The surgical preparation and intraoperative management of this conduit artery may affect its early and long-term patencies. We investigated the effects of the colloidal Biseko^® and 5% albumin solutions as well as the crystalloid physiological saline (0.9% NaCl) and Bretschneider solutions on the contractile and relaxing capacities of isolated human radial artery grafts. Method: Radial artery segments were harvested using the technique with an ultrasonic scalpel, and 2.5–3 mm rings were obtained from the proximal part of the artery. Arterial rings were stored in Biseko^® or 5% albumin solutions and in 0.9% NaCl or Bretschneider solutions for 45 min. Isometric tensions of radial arteries obtained from 26 patients were measured in isolated organ baths. Contractions were induced by 0.31 µmol L^–1 5-hydroxytryptamine and 10 µmol L^–1 noradrenaline. Endothelium-dependent relaxations were induced by 10 µmol L^–1 acetylcholine and 1 µmol L^–1 bradykinin as well as the endothelium-independent relaxations by 10 µmol L^–1 glyceryl trinitrate and 100 µmol/l papaverine. Results: Contractions of radial arteries induced by 5-hydroxytryptamine were significantly lower following storage in Biseko^® solution (12.6 ± 4.4 mN) than in 5% albumin (37.9 ± 13.0 mN, p = 0.03) or in 0.9% NaCl solution (35.9 ± 11.9 mN, p = 0.04). Noradrenaline-induced contractions of the arteries were also diminished in Biseko^® solution compared to those stored in 5% albumin (32.9 ± 6.2 mN vs 49.2 ± 6.4 mN, p = 0.01). No significant differences in relaxations were obtained between the two crystalloid and the two colloidal solutions using endothelium-dependent and independent vasorelaxants. Conclusion: Our results suggest that storage of radial artery in Biseko^® colloidal solution before coronary artery bypass grafting decreases the sensitivity of the graft to vasoconstriction, thereby decreasing the risk of intra/perioperative graft failure.

Key Words: Radial artery • Biseko^® solution • Graft storage • Vasoconstriction