Files in this Data Supplement:

• Video 1 - Video 1 (.rv format). Procedures for the transplantation of autologous dermal fibroblast sheets following lung puncture. After thoracotomy was performed, an incision 15 mm-in diameter and 10 mm-in depth is made to create a lung puncture model. Air leaks were subsequently confirmed by the presence of air bubbles on the lung surface. A 25 cm² autologous skin fibroblast sheet is harvested from a temperature-responsive culture dish and transplanted using a square-shaped supporter. After allowing 5 min. for the cell sheet to attach to the pleural surface, the supporter is carefully removed. A second autologous cell sheet is then transplanted in the same fashion. At inspiratory pressures of 20-25 cm H₂O, which are used to clinically assess air leaks after pulmonary resection, the cell sheets act to immediately seal the lung punctures. When pressures are raised above 30 cm H₂O recurrence of air leaks occurs. However, when pressures are again reduced to 25 cm H₂O, all air leaks are subsequently eliminated.
• Video 1 - Video 1 (.mov format). Procedures for the transplantation of autologous dermal fibroblast sheets following lung puncture. After thoracotomy was performed, an incision 15 mm-in diameter and 10 mm-in depth is made to create a lung puncture model. Air leaks were subsequently confirmed by the presence of air bubbles on the lung surface. A 25 cm² autologous skin fibroblast sheet is harvested from a temperature-responsive culture dish and transplanted using a square-shaped supporter. After allowing 5 min. for the cell sheet to attach to the pleural surface, the supporter is carefully removed. A second autologous cell sheet is then transplanted in the same fashion. At inspiratory pressures of 20-25 cm H₂O, which are used to clinically assess air leaks after pulmonary resection, the cell sheets act to immediately seal the lung punctures. When pressures are raised above 30 cm H₂O recurrence of air leaks occurs. However, when pressures are again reduced to 25 cm H₂O, all air leaks are subsequently eliminated.
• Video 2 - Video 2 (.rv format). Autologous dermal fibroblast sheets can permanently seal lung air leaks. Four weeks after the transplantation of two autologous fibroblast sheets to seal lung punctures, no air leaks are observed. Autologous dermal fibroblast sheets maintained stable closure of the pleural defects without any lung constriction. The transplanted cell sheets were also flexible to allow for lung expansion and contraction during respiration, even under mechanical ventilation.
• Video 2 - Video 2 (.mov format). Autologous dermal fibroblast sheets can permanently seal lung air leaks. Four weeks after the transplantation of two autologous fibroblast sheets to seal lung punctures, no air leaks are observed. Autologous dermal fibroblast sheets maintained stable closure of the pleural defects without any lung constriction. The transplanted cell sheets were also flexible to allow for lung expansion and contraction during respiration, even under mechanical ventilation.